The investigation of the changes in the surface glycoconjugates using two different spheroid models of breast cancer cells and availability assessment of these spheroid models for rapid diagnosis

The importance of early cancer diagnosis has led to development many different diagnostic methods. In this context, the studies investigating presence and amount sugar residues use as indicators in identification cell type have become prominent. present study, sialic acids found on membrane surfaces ER (+) MCF-7 (-) MDA-MB-231 breast lines were labeled using three-dimensional (3D) culture (Spheroid) model closest method patient sample, thus its natural environment, among These units that play a role regulation important immune characteristics such recognition, binding metastasis made visualizable by applying fluorescent-labeled lectins FITC-(Wheat Germ Agglutinin) specifically acid (GlcNAc, Neu5Ac) including particularly ß-GlcNAc FITC-(Maackia Amurensis-Lectin-1) Galß4GlcNAc acids. glycan with FITC- (Wheat radiation intensities analyzed relatively. two cultures compared respect change amounts containing α-2,3- α-2,6 bonds lectins. we performed precise, accurate rapid determination content surface means carried out relative comparison between micrographs.